Hydrolysis of sphingomyelin liposomes by sphingomyelinase
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چکیده
منابع مشابه
Kinetic steps for the hydrolysis of sphingomyelin by Bacillus cereus sphingomyelinase in lipid monolayers.
The sphingomyelinase (Sphmase) activity degrading sphingomyelin (Sphm) monolayers shows a slow-reaction latency period before exhibiting constant rate catalysis. These two kinetic regions are regulated independently by the lateral surface pressure and by lipids that are biomodulators of cell function such as ceramide, glycosphingolipids, fatty acids, and lysophospholipids. Knowledge of the inte...
متن کاملHis151 and His296 are the acid-base catalytic residues of Bacillus cereus sphingomyelinase in sphingomyelin hydrolysis.
Bacillus cereus sphingomyelinase belongs to the Mg(2+)-dependent neutral sphingomyelinase, which hydrolyses sphingomyelin to phosphocholine and ceramide, and acts as an extracellular hemolysin. The triplet residues, His151-Asp195-His296, of the enzyme are highly conserved among bacterial and mammalian Mg(2+)-dependent neutral sphingomyelinases. The triplet residues converge on the active-site p...
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Apoptosis, a form of cell death that is coordinated by a set of defined biochemical pathways, exists throughout the animal kingdom. Its critically important roles in development, homeostasis, and disease have made this a topic of intensive research and sometimes even more intensive controversy. One of these areas of controversy concerns the role of ceramide, produced by the hydrolysis of sphing...
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Sphingomyelin in mixed dispersion with bile salts was hydrolysed by the solubilized sphingomyelinase of rat brain lysosomes. In parallel studies, physical properties of these dispersions were determined. The kinetic curves that described the rate of hydrolysis as a function of increasing concentrations of bile salt were multiphasic. A region of very low activity was followed by an ascending por...
متن کاملSphingomyelin Liposomes Containing Porphyrin-phospholipid for Irinotecan Chemophototherapy
Porphyrin-phospholipid (PoP) liposomes can entrap anti-cancer agents and release them in response to near infrared (NIR) light. Doxorubicin, when remotely loaded via an ammonium sulfate gradient at a high drug-to-lipid ratio, formed elongated crystals that altered liposome morphology and could not be loaded into liposomes with higher PoP content. On the other hand, irinotecan could also be remo...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1973
ISSN: 0014-5793
DOI: 10.1016/0014-5793(73)80670-2